Fig. 1

CBR4 is downregulated in CRC. (A) CBR4 was down-regulated in colorectal cancer (CRC) compared to normal tissues, as indicated by the TCGA database (p = 2.261700E-02). (B) The expression of CBR4 decreased with increasing tumor stage in the TCGA database (Stage 1 vs. Stage 4, p = 1.285680E-02). (C) CBR4 protein levels in CRC tissues were lower than those in the control group, according to the CPATC database (p = 2.000459E-02). (D) CBR4 protein levels decreased as tumor stage increased, as reported in the CPATC database (p > 0.05). (E) Low expression of CBR4 was associated with shorter survival probabilities in CRC cases, as shown in the TCGA database. (F-G) Quantitative PCR and western blot analyses were conducted to assess the RNA and protein levels of CBR4 in CRC and normal tissues. (H-I) The RNA and protein levels of CBR4 decreased as tumor metastasis progressed. (J) Levels of CBR4 protein were compared between various CRC cell lines and normal cells. (K) Immunohistochemical staining of CBR4 was performed on CRC tumor tissues (T), paracancerous tissues (NT), metastatic tumors (M), and non-metastatic tumors (NM) from patients, with a magnification of ×100. (*P < 0.05; ***P < 0.001)