Fig. 6
From: Carbonyl reductase 4 suppresses colorectal cancer progression through the DNMT3B/CBR4/FASN/mTOR axis
DNMT3B regulates CBR4 via methylation. (A) The methylation level of CBR4 in CRC and normal cells (p = 3.231300E-04). (B) The methylation level of CBR4 in different stages of CRC(Stage1-vs-Stage3 p = 2.749800E-02). (C-E) The mRNA and protein levels of CBR4 in RKO and SW480 cells after treatment with DNA methylation inhibitor 5aza. (F) The levels of mTOR/p-mTOR in RKO and SW480 cells after treatment with 5aza. (G-H) mRNA levels of DNMT3A, DNMT3B and DNMT1 in knockdown and control CRC cells. (I-K) mRNA and protein levels of CBR4 in DNMT3A, DNMT3B, DNMT1 knockdown and control CRC cells. (L) mRNA levels of DNMT3B in over-expression and control CRC cells. (M-N) mRNA and protein levels of CBR4 in DNMT3B over-expression and control CRC cells. ( ***P < 0.001)