Fig. 2

TCDCA arrested cell cycle of GBM cells at G2/M phase. A Representative flow cytometry results of cell cycle (A) and quantitative analysis in C6 cells after 200 µM TCDCA treatment for 24 h (B, n = 3/group, t-test). C, D Representative flow cytometry results of cell cycle (C) and quantitative analysis in U251 cells after TCDCA treatment for 24 h (D, n = 3/group, t-test). E The expression of Cyclin B1 in C6 cells was detected after treated with 200 μM TCDCA for 24 h by western blot. F Quantification analysis of the relative Cyclin B1 level as shown in (E) (normalized to control, n = 4/group, Mann–Whitney test). G The expression of Cyclin B1 in U251 cells was detected after treated with 200 μM TCDCA for 24 h by western blot. H Quantification analysis of relative Cyclin B1 level as shown in (G) (normalize to control, n = 4/group, Mann–Whitney test). Data were mean + SEM. ^*P < 0.05, ^**P < 0.01