Fig. 5

TCDCA suppressed GBM cells through inducing ferroptosis. A Western blot detected the expression of GPX4 in U251 cells treated with 200 μM TCDCA. B Quantification of the relative level of GPX4 as shown in (A) (n = 4 per group, t-test). C Representative results of ROS analysis in U251 cells after 200 μM TCDCA treatment. D Quantitative analysis of ROS in U251 cells as shown in (C) (n = 3/group, t-test). E The cell viability of U251 cells treated with different concentrations of FER-1 and 200 μM TCDCA detected by CCK-8 assays (n = 4/group, two-way ANOVA, Sidak's test was performed for the multiple comparison). F Representative images of U251 cells treated with 200 μM TCDCA and 1.25 μM FER-1 in wound healing assays. Phase-contrast images were acquired at 0 h, 24 h after scratching and representative images of three independent experiments were shown. Scale bars, 200 µm. G Quantitative analysis of the numbers of migrated U251 cells (n = 15/group, two-way ANOVA, Sidak's test was performed for the multiple comparison) and cells counted in representative high-power fields per Transwell plate. H Representative images of U251 cells treated with 200 μM TCDCA and 1.25 μM FER-1 for 24 h in Transwell migration assay. Scale bar, 100 µm. I Quantitative analysis of the numbers of migrated U251 cells and cells counted in representative high-power fields per Transwell plate (n = 15/group, two-way ANOVA, Sidak's test was performed for the multiple comparison). Data were mean + SEM. ^*P < 0.05, ^**P < 0.01