Fig. 6

TCDCA induced ferroptosis of GBM cells through HMGCS1-HMGCR-GPX4 pathway. A Western blot detected the expression of HMGCS1, HMGCR and GPX4 in U251 cells treated with 200 μM TCDCA and SIN. B–D Quantification of the relative level of HMGCR (B), HMGCR (B) (normalized to control, n = 5/per group, two-way ANOVA, Sidak's test) and GPX4 (C) (normalized to control, n = 6/per group, two-way ANOVA, Sidak's test) and HMGCS1 (D) (n = 6/per group, two-way ANOVA, Sidak's test) as shown in (A). E Representative results of ROS analysis in U251 cells after 200 μM TCDCA and 0.5 μM SIN treatment. F Quantitative analysis of ROS in U251 cells (n = 3/group, two-way ANOVA, Sidak's test) as shown in (E). G Quantification of the relative level of GSH (n = 3/group, two-way ANOVA, Sidak's test). Data were mean + SEM. ^*P < 0.05, ^**P < 0.01