Fig. 1

Expression pattern of Swi/Snf-deficient ECA. (A) Representative images of Swi/Snf complex subunits staining patterns in ECA. (a) Diffuse nuclear staining of SMARCA2 (IHC, 20×); (b) Complete deficiency of SMARCA2 expression, with positive stromal cells as an internal control (IHC, 20×); (c) ‘checkerboard’ staining pattern (SMARCA2, IHC, 20×); (d) Reduced expression (ARID1B, IHC, 20×); (e-f) Tumor heterogeneity deficiency of SMARCA4 (HE, IHC, 20×, intact expression in the bottom area showing gland-forming carcinoma cells, deficiency expression in the top area showing solid growing tumor cells). (B) Protien-deficiency of ARID1A, SMARCA2, and SMARCA4 in ECA. First line: (a) ECA (HE, ×10) with deficiency of ARID1A expression (b) (IHC, 20×) in gastric type ECA. Second line: (c) ECA (HE, ×10) with deficiency of SMARCA2 expression (d) (IHC, 20×). Third line: (e) ECA (HE, ×10) with deficiency of SMARCA4 expression (f) (IHC, 20×). (C) Statistical picture of Swi/Snf-deficient ECA. (a) Frequencies of the deficiency of ARID1A, SMARCA2, and SMARCA4, (b) Swi/Snf-deficient ECA (ARID1A, SMARCA2, or SMARCA4) was significantly correlated with NHPVA (15.2% and 5.2%, ‘*’ represents P < 0.05). (D) The Venn diagram showed concurrent deficiency of ARID1A, SMARCA2, and SMARCA4