Fig. 3

Swi/Snf-deficient ECA showed a significant correlation with dMMR and immune microenvironment. (A) Representative images under the microscope showed that deficiency of ARID1A, SMARCA2, and SMARCA4 combined with dMMR. (a-e) Deficiency of ARID1A, SMARCA2, MLH1, and PMS2 (N = 1, HE and IHC, 20×); (f-j) deficiency of ARID1A, SMARCA2, MSH2, and MSH6 (N = 5, HE and IHC, 20×); (k-o) deficiency of ARID1A, SMARCA4, and MSH6, and the intact expression of MSH2 (n) as contrast (N = 1). (B) Swi/Snf- deficient ECA was significantly correlated with dMMR, including ARID1A-deficient, and SMARCA2-deficient respectively, except SMARCA4-deficient, which shows no statistical difference. (C) Representative H&E and IHC images under the microscope showed different expressions compared Swi/Snf-deficient with Swi/Snf-intact ECA. (D) The density of CD3, CD8, CD38, CD56, and CD68 in Swi/Snf-deficient ECA was higher, and the positive rate PD-L1 (CPS) was also significantly higher (The density of IHC-positive immunocytes underwent natural logarithmic conversion. ‘*’ means P < 0.05; ‘* *’ means P < 0.01; ‘* * *’ means P < 0.001; ns, no significance)