Fig. 1

Identification of USP24 as a regulator for YAP1 in HCC. A Analysis and generation of heat maps of CNV values of 56 USPs in the STAD dataset using cBioPortal. B After silencing USPs (CNV≥2%) in Bel-7402 cells, YAP1/TEAD4-driven transcriptional activity was accessed, and it is represented by the ratio of 8×GTIIC-luciferase activity with 8 TBSs to GTIIC luciferase activity with 8 mutant TBSs. C Bel-7402 and Huh-7 cells were treated with increasing concentrations of WP1130 for 12 h, and the indicated proteins were measured by western blotting. D USP24 was depleted by shRNAs (shUSP24-1 and shUSP24-2) in HCC cells and the indicated proteins were examined by western blotting. Control shRNA (shNC) was used as negative control. E qRT-PCR analysis of the indicated genes in Bel-7402 and Huh-7 cells with USP24 knockdown. F Increasing amounts of Flag-USP24 plasmids were co-transfected with Myc-YAP1 plasmid into HEK293T cells and the indicated proteins were examined by western blotting. G HEK293T cells were transfected with Flag-USP24 or vector plasmids. A CHX chase experiment was performed and Myc-tagged YAP1 protein was determined by western blotting (upper panel). The lower panel shows the relative protein amounts of different groups. H Bel-7402 cells stably expressing control shRNA or USP24 shRNAs were treated with or without CHX (20 mg/mL) and harvested at the indicated times, and indicated proteins was determined by western blotting (upper panel). The lower panel shows the relative protein amounts of different groups. Data are presented as mean ± SD, and P values were calculated using Student t test. ^*P < 0.05 and ^**P < 0.01