Fig. 3

USP24 deubiquitinates YAP1. A HEK293T cells were co-transfected with the specified plasmids. After 48 h of transfection, cell extracts were prepared for immunoprecipitation assays with anti-Myc followed by western blotting with anti-HA antibody. B HEK293T cells were co-transfected with the indicated plasmids, treated with or without WP1130 (10 µM) for 8 h before cells were harvested. Further, cellular extracts were prepared for immunoprecipitation assays with anti-Myc followed by western blotting with anti-HA antibody. C HEK293T cells stably expressed shRNAs specifically against USP24 and co-transfected with the indicated plasmids. Cellular extracts were immunoprecipitated with anti-Myc followed by western blotting with anti-HA antibody