Fig. 9

Consistent effects of moxibustion and kdm4d^−/− on colonic lipid metabolism in CAC. A 3D-PCA diagram of the WT-NC, WT-CAC and KO-CAC groups. B Heatmap of differential lipids in the colons of the WT-NC, WT-CAC, and KO-CAC. C Volcano plot of colonic differential lipids between the WT-CAC group and the KO-CAC group. The red dots represent lipids upregulated in the KO-CAC group compared with the WT-CAC group, and the blue dots represent lipids downregulated in the KO-CAC group compared with the WT-CAC group. D Venn diagram of lipids significantly downregulated in the WT-CAC group compared with the WT-NC group and significantly upregulated in the KO-CAC group compared with the WT-CAC group. E Venn diagram of lipids significantly upregulated in the WT-CAC group compared with the WT-NC group and significantly downregulated in the KO-CAC group compared with the WT-CAC group. F 3D-PCA diagram of the KO-NC, KO-CAC and KO-MOX groups. G Heatmap of differential lipids in the colons of the KO-NC, KO-CAC, and KO-MOX groups. H Comparison of SM (d18:1/26:0) content in the colons of the mice. I Comparison of TAG58:7(18:0) content in the colons of the mice. J Spearman’s correlation analysis between SM(d18:1/26:0) and KDM4D mRNA. K Spearman’s correlation analysis between SM(d18:1/26:0) and β-Catenin mRNA. L Spearman’s correlation analysis between TAG58:7(18:0) and KDM4D mRNA. M Spearman’s correlation analysis between TAG58:7(18:0) and β-Catenin mRNA. N Graphical example of how moxibustion affects colon KDM4D/β-Catenin and lipid metabolism. n = 6, ^*P<0.05, ^**P<0.01, ^***P<0.001