Fig. 9

NRP1 Knockdown suppresses ccRCC cell migration, invasion, EMT, and in vivo tumor growth. A, B Transwell migration and invasion assays showing significantly reduced migratory and invasive capacities post-NRP1 knockdown. C, D Quantitative analysis of migration and invasion. E, F Wound-healing assay demonstrating reduced cell motility with NRP1 knockdown. G GSEA plot showing the association between NRP1 and EMT. H Western blot analysis indicating EMT inhibition, with increased E-cadherin and decreased N-cadherin and Vimentin expression. I Representative images of tumors from sh-NC and shNRP1 xenograft models, showing reduced tumor sizes in the shNRP1 group. J Tumor growth curves showing significantly lower tumor volume in the NRP1 knockdown group. K Tumor weight measurements indicating reduced tumor mass post-knockdown. L H&E staining and IHC staining results showing reduced NRP1, Ki67 and CD31 expression, decreased Vimentin, and increased TUNEL-positive cells