Fig. 3
From: SP1 promotes triple-negative breast cancer progression by targeting USP5
USP5 is regulated by transcription factor SP1 that binds to its promoter in TNBC cells. (A) The diagram showed the SP1 binding sites located in the promoter region of USP5. (B) MDA-MB-231 cells were co-transfected with pGL3‑basic, pGL3-USP5-WT, or pGL3-USP5-MUT promoter vector and pLV-Ctrl or pLV-SP1 expression vector, and the luciferase activity was determined using a dual luciferase reporter assay. ***P < 0.001, **P < 0.01, *P < 0.05. (C, D) ChIP-qPCR analysis of SP1 binding to USP5 promoter in MDA-MB-231 cells. **P < 0.01. (E, F) The USP5、SP1 mRNA (E) and protein (F) expression in control and SP1 knockdown MDA-MB-231 cells. ***P < 0.001, **P < 0.01