Fig. 2

Glutamine enhances glycolytic capacity. Cells were incubated as indicated for four days and then subjected to the GlycoStress assay. The ExtraCellular Acidification Rate (ECAR) measured (A, K562; B, KCL22) is shown as complete patterns obtained from representative Seahorse runs (left panels) or as the average of independent measures (middle and right panels). The correlation between EACR and intracellular and extracellular lactate production is shown in Supplementary Fig. 1E. The latter data are expressed as the mean ± SEM of the results from at least three independent experiments, each consisting of cell plating into six Seahorse microplate wells (n = 3)