Fig. 3
The lack of oxygen affects cell metabolic demand. Cells were incubated as indicated for 4 days and then subjected to MitoStress or Mito Fuel Flex assays. The Oxygen Consumption Rate (OCR) measured by the mitostress test (A, K562; B, KCL22) is shown as complete patterns obtained from representative Seahorse runs (left panels) or as the average of independent measures (middle and right panels). The Fuel Oxidation Dependency (C) was calculated using the following equation: \(\:\text{D}\text{e}\text{p}\text{e}\text{n}\text{d}\text{e}\text{n}\text{c}\text{y}\:\text{\%}=\:\left[\frac{\text{B}\text{a}\text{s}\text{e}\text{l}\text{i}\text{n}\text{e}\:\text{O}\text{C}\text{R}-\text{T}\text{a}\text{r}\text{g}\text{e}\text{t}\:\text{I}\text{n}\text{h}\text{i}\text{b}\text{i}\text{t}\text{o}\text{r}\:\text{O}\text{C}\text{R}}{\text{B}\text{a}\text{s}\text{e}\text{l}\text{i}\text{n}\text{e}\:\text{O}\text{C}\text{R}-\text{A}\text{l}\text{l}\:\text{i}\text{n}\text{h}\text{i}\text{b}\text{i}\text{t}\text{o}\text{r}\text{s}\:\text{O}\text{C}\text{R}}\right]*100\). Only the oxidation dependence of FA was statistically significant. Averaged data are expressed as the mean ± SEM of results from at least three independent experiments, each consisting of cell plating into six Seahorse microplate wells (n = 3)