Fig. 6

CD36-mediated FA uptake induces a block of de novo FA synthesis. K562 and KCL22 cells were incubated for 4 days as indicated. (A) CD36 expression was measured by flow cytometry. Glc(-) Gln(+) in hypoxia was compared only to Glc(+) Gln(+) and Glc(+) Gln(-) in the same oxygen condition, and versus Glc(-) Gln(+) in normoxia (B) Total cell lysates were subjected to immunoblotting to determine the expression levels of FA-synthase and BCR::ABL1. The loading of equal amounts of vinculin protein was confirmed by immunoblotting and the average densitometric values of bands from three independent experiments, obtained using ImageJ software, are reported in Supplementary Fig. 1A. Data reported in (A) are expressed as the mean ± SD of the data from three independent experiments (n = 3)