Fig. 7

FA interfere with BCR::ABL1 expression and the maintenance of stem cell potential. K562 and KCL22 cells were incubated as indicated for 4 days and treated from time zero of incubation with BSA-Palmitate (ratio 1:8–100 µM) in the presence of Glc only (A) or with SSO (150 µM) in the presence of Gln only (B, C). Total cell lysates were subjected to immunoblotting to determine the expression levels of BCR::ABL1 and phospho-Crkl, as well as vinculin, to confirm the loading of equal amounts of protein. The average densitometric values of bands from three independent experiments, obtained using ImageJ software, are reported in Supplementary Figs. 1B, C, and D, respectively. In (D), cells incubated for 4 days in low oxygen (LC1) in the absence or presence of SSO from time 0 of LC1 incubation were replated into growth-permissive cultures established in complete growth medium and incubated in normoxia (LC2) in the absence or presence of etomoxir from time 0 of LC2 incubation. Trypan blue-negative cells were counted after 5 (left panels) or 13 (right panels) days of incubation in LC2 medium. Data are expressed as the mean ± SEM of data from four independent experiments (n = 3)