Fig. 8

CML cells stimulate BM adipocytes to secrete FA. Total RNA isolated using Tri Reagent from HS27A cells previously differentiated into adipocytes, alone (A) or co-cultured with CML cells (C), was subjected to RT, and qPCR was performed for FAPB4 and PPARγ normalized by comparison with three different housekeeping genes (GAPDH, 18 S, and ACTB). (B) Conditioned media from differentiated HS27A cells were collected and the neutral lipid content was evaluated by Nile Red staining. All experiments were performed with cells incubated in low oxygen for four days. Data are expressed as the mean ± SD of data from at least three independent experiments (n = 3)