Fig. 4
FBXL18 positively regulated JNK signaling pathway in EC. (A) Volcano plot of FBXL18-related differentially expressed genes based on the RNA sequencing data of EC samples with high- and low-FBXL18 expression. (B-C) GO and KEGG enrichment analyses of FBXL18-related differentially expressed genes. (D-E) The results of GSEA revealed that MAPK and ubiquitin-mediated proteolysis were markedly enriched in FBXL18High group. (F-T) The Pearson’s correlation analyses of FBXL18 and genes of the MAPK signaling pathway. (U-W) The protein levels of JNK, p-JNK, c-JUN, and p-c-JUN in EC cells after silencing FBXL18 were determined by western blot assay, and quantitative analysis. (X-Z) The protein levels of JNK, p-JNK, c-JUN, and p-c-JUN in EC cells after overexpressing FBXL18 were measured by western blot assay, and quantitative analysis. *P < 0.05; **P < 0.01; ***P < 0.001