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Fig. 2 | Cancer Cell International

Fig. 2

From: OTUD3 inhibits breast cancer cell metastasis by regulating TGF-β pathway through deubiquitinating SMAD7

Fig. 2

Expression levels of OTUD3 and SMAD7 and the correlation of both in cells and tissues. A The protein levels of OTUD3 and SMAD7 in MCF10A and BRCA cells. B-C The expression levels of mRNA of OTUD3 (B) and SMAD7 (C) in MCF10A and BRCA cells. D-F OTUD3 and SMAD7 antibodies were successively used to stain the breast cancer tissue microarray with immunohistochemical staining and correlation analysis. D Representative pictures. E Percentage of high or low SMAD7 expression in tissues expressing high or low OTUD3. F The scatterplot showed that the expression levels of OTUD3 and SMAD7 were positively correlated (p < 0.001). G In Kaplan-Meier Plotter database, the recurrence-free survival (RFS) was positively correlated with the expression of OTUD3 (p = 0.0072). H Knockdown (#1,#2) of OTUD3 in MDA-MB-231 and SK-BR-3 cells. Western blotting was used to detect the protien levels of OTUD3 and SMAD7. Co.sh, non-targeting shRNA

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Cancer Cell International

ISSN: 1475-2867

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