Correction: TEAD4 overexpression promotes epithelial-mesenchymal transition and associates with aggressiveness and adverse prognosis in head neck squamous cell carcinoma

Correction: Cancer Cell International (2018) 18:178

https://doiorg.publicaciones.saludcastillayleon.es/10.1186/s12935-018-0675-z.

In this article [1], the author would like to correct the mistake with the Western blot image in Fig. 6a, g. Based on the retrieved original datasets, they confirmed that the TEAD4 and GAPDH used in both Fig. 6g and a come from the same single-round WB experiment in the same HN6 cell lysates which were included during figure preparation. Thus, they appeared to be duplicated.

The incorrect and Corrected Fig. 6 and revised figure legend have been shown below:

Incorrect Fig. 6

Fig. 6

Ectopic TEAD4 overexpression induces EMT-like changes in HNSCC cells. a TEAD4 overexpression was confirmed by western blot in cellular lysates from 293T and HN6 cells infected with TEAD4 cDNA plasmid. Representative images of western blot (WB) were shown from 3 independent experiments. b Cell proliferation was remarkably promoted following TEAD4 overexpression by CCK-8 viability assay. c The potentials of colony formation were significantly promoted in TEAD4-overexpressed cells as compared to control. d Enforced TEAD4 overexpression resulted in EMT-like morphological changes from cobble-like to spindle-like appearance under phase contrast microscopy. e, f The cell motilities and invasion were remarkably enhanced after TEAD4 overexpression as gauged by wound healing (e) and transwell-invasion assay (f). Measurements of wound healing was performed at 6 and 12 h after cell scratching while measurements of transwell assays were done at 12 h after cell seeding. g The abundance of EMT markers E-cadherin, N-cadherin, Vimentin and Snail following TEAD4 overexpression were assayed by western blot (left panel).These protein blotting in Fig. 6a and g was performed in single-round western blotting experiments with the same cell lysates. Same GAPDH blotting image was used in both Fig. 6a and g. E-cadherin and vimentin expression was probed by immunofluorescent staining in TEAD4-overexpressed and control cells (right panel). The Scale bar: 100 μm. Representative images are shown. Data showed here are mean ± SD from three independent experiments. **P < 0.01, Student-t test

Correct Fig. 6

Fig. 6

Ectopic TEAD4 overexpression induces EMT-like changes in HNSCC cells. a TEAD4 overexpression was confirmed by western blot in cellular lysates from 293T and HN6 cells infected with TEAD4 cDNA plasmid. Representative images of western blot (WB) were shown from 3 independent experiments. b Cell proliferation was remarkably promoted following TEAD4 overexpression by CCK-8 viability assay. c The potentials of colony formation were significantly promoted in TEAD4-overexpressed cells as compared to control. d Enforced TEAD4 overexpression resulted in EMT-like morphological changes from cobble-like to spindle-like appearance under phase contrast microscopy. e, f The cell motilities and invasion were remarkably enhanced after TEAD4 overexpression as gauged by wound healing (e) and transwell-invasion assay (f). Measurements of wound healing was performed at 6 and 12 h after cell scratching while measurements of transwell assays were done at 12 h after cell seeding. g The abundance of EMT markers E-cadherin, N-cadherin, Vimentin and Snail following TEAD4 overexpression were assayed by western blot (left panel).These protein blotting in Fig. 6a and g was performed in single-round western blotting experiments with the same cell lysates. Same GAPDH blotting image was used in both Fig. 6a and g. E-cadherin and vimentin expression was probed by immunofluorescent staining in TEAD4-overexpressed and control cells (right panel). The Scale bar: 100 μm. Representative images are shown. Data showed here are mean ± SD from three independent experiments. **P < 0.01, Student-t test