Fig. 2
From: Brachyury promotes proliferation and migration of colorectal cancer cells by targeting MMP14
BRY suppression reduces HCT116 and SW480 cell proliferation and migration. (A) qRT-PCR analysis (n = 3) of BRY expression in HCT116 and SW480 cells transfected with anti-BRY siRNAs (si-BRY-1# or si-BRY-2#) or control siRNA (si-NC). (B) Western blot analysis of BRY expression in HCT116 and SW480 cells transfected with si-NC, si-BRY-1#, or si-BRY-2#. Similar results were observed from three independent experiments. (C–D) CCK-8 analysis of HCT116 (C) and SW480 (D) CRC cells (n = 6). (E) Colony formation analysis of the proliferation of si-BRY-transfected CRC cells (n = 3). (F) Quantitative analysis of (E). (G) Transwell assays on CRC cells (n = 3, scale bar: 100 μm). (H) Quantitative analysis of (G). Data are shown as means ± SD; **p < 0.01, ***p < 0.001; one-way ANOVA