Fig. 4

BRY silencing inhibited HCT116 cell proliferation in vivo. (A) Subcutaneous tumor volumes in nude mice were measured at three-day intervals following injection (n = 5). (B) After 12 days, the tumors were harvested and photographed using a smartphone. (C) Tumor weights from the empty vector and sh-BRY groups were measured (n = 5). (D) Western blot analysis of BRY expression in the empty vector and sh-BRY groups. Similar results were observed from three independent experiments. (E) Immunofluorescence analysis of Ki-67 expression in the empty vector and sh-BRY groups (scale bar: 50 μm, n = 5). (F) Quantitative analysis of (E). Data are shown as means ± SD; ^*p < 0.05, ^**p < 0.01; Student’s t-test